Chlamydomonas reinhardtii      Asp-Based PSP/FCP


※ FCP family introduction

    FCP/SCP phosphatase belongs to a family of phosphatase which uses an Asp nucleophile for catalysis. FCP/SCP is characterized by its phosphatase activity towards the CTD of the largest subunit of RNA polymerase II (1). At least seven members have been identified in human genome and FCP/SCP phosphatases share a conserved catalytic signature, which is known as DXDXT/V (2). Family member FCP1 is conserved among all eukaryotes and plays an important role in cell survival in budding and fission yeasts. SCP1 preferentially dephosphorylates Ser5 in CTD domain of RNA polymerase II and plays a role in the regulation of gene expression by controlling the transition from initiation/capping to processive transcript elongation (3). In addition, SCP1 also acts as a key regulator that functions in silencing neuronal gene expression (4).

Reference
1. Shi, Y. (2009) Serine/threonine phosphatases: mechanism through structure. Cell, 139, 468-484. PMID: 19879837
2. Moorhead, G.B., Trinkle-Mulcahy, L. and Ulke-Lemee, A. (2007) Emerging roles of nuclear protein phosphatases. Nat Rev Mol Cell Biol, 8, 234-244. PMID: 17318227
3. Yeo, M., Lin, P.S., Dahmus, M.E. and Gill, G.N. (2003) A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5. J Biol Chem, 278, 26078-26085. PMID: 12721286
4. Yeo, M., Lee, S.K., Lee, B., Ruiz, E.C., Pfaff, S.L. and Gill, G.N. (2005) Small CTD phosphatases function in silencing neuronal gene expression. Science, 307, 596-600. PMID: 15681389


There are 11 genes.  Reviewed (0 or Unreviewed (11

No.StatusiEKPD IDEnsemble Gene IDUniProt AccessionGene Name
1
iEKPD-Chr-0848
CHLREDRAFT_187332
A8HYX3
CGL55
2
iEKPD-Chr-0597
CHLREDRAFT_111940
A8I2V5
CHLREDRAFT_111940
3
iEKPD-Chr-0569
CHLREDRAFT_115803
A8ITD7
CHLREDRAFT_115803
4
iEKPD-Chr-0590
CHLREDRAFT_118402
A8J044
CHLREDRAFT_118402
5
iEKPD-Chr-0596
CHLREDRAFT_141879
A8I4K4
CHLREDRAFT_141879
6
iEKPD-Chr-0593
CHLREDRAFT_142839
A8I7G0
CHLREDRAFT_142839
7
iEKPD-Chr-0571
CHLREDRAFT_149314
A8J204
CHLREDRAFT_149314
8
iEKPD-Chr-0588
CHLREDRAFT_149388
A8J274
CHLREDRAFT_149388
9
iEKPD-Chr-0595
CHLREDRAFT_187551
A8I7G3
CHLREDRAFT_187551
10
iEKPD-Chr-0579
CHLREDRAFT_193029
A8J8H4
CHLREDRAFT_193029
11
iEKPD-Chr-0612
CHLREDRAFT_169672
A8IJZ0
TIM50